Skip to Main content Skip to Navigation
Journal articles

Rapid and robust optogenetic control of gene expression in Drosophila

Abstract : Deciphering gene function requires the ability to control gene expression in space and time. Binary systems such as the Gal4/UAS provide a powerful means to modulate gene expression and to induce loss or gain of function. This is best exemplified in Drosophila, where the Gal4/UAS system has been critical to discover conserved mechanisms in development, physiology, neurobiology, and metabolism, to cite a few. Here we describe a transgenic light-inducible Gal4/UAS system (ShineGal4/UAS) based on Magnet photoswitches. We show that it allows efficient, rapid, and robust activation of UAS-driven transgenes in different tissues and at various developmental stages in Drosophila. Furthermore, we illustrate how ShineGal4 enables the generation of gain and loss-of-function phenotypes at animal, organ, and cellular levels. Thanks to the large repertoire of UAS-driven transgenes, ShineGal4 enriches the Drosophila genetic toolkit by allowing in vivo control of gene expression with high temporal and spatial resolutions.
Document type :
Journal articles
Complete list of metadata

https://hal.sorbonne-universite.fr/hal-03474714
Contributor : HAL Sorbonne Université 5 Gestionnaire Connect in order to contact the contributor
Submitted on : Friday, December 10, 2021 - 12:43:37 PM
Last modification on : Sunday, June 26, 2022 - 3:24:38 AM
Long-term archiving on: : Friday, March 11, 2022 - 7:07:45 PM

File

1-s2.0-S1534580721009400-main....
Publication funded by an institution

Identifiers

Citation

Florencia Di Pietro, Sophie Herszterg, Anqi Huang, Floris Bosveld, Cyrille Alexandre, et al.. Rapid and robust optogenetic control of gene expression in Drosophila. Developmental Cell, Elsevier, 2021, ⟨10.1016/j.devcel.2021.11.016⟩. ⟨hal-03474714⟩

Share

Metrics

Record views

94

Files downloads

30